Circular RNA circMYL1 Inhibit Proliferation and Promote Differentiation of Myoblasts by Sponging miR-2400
نویسندگان
چکیده
Circular RNAs (circRNAs) are a class of endogenous non-coding (ncRNAs) involved in regulating skeletal muscle development by sponging miRNAs. In this study, we found that the circMYL1 expression was down-regulated during myoblast proliferation, while gradually up-regulated differentiation. The potential role identified proliferation bovine through mRNA and protein marker genes (PCNA, CyclinD1, CDK2), cell counting kit-8 assay, flow cytometry analysis, 5-ethynyl 2′-deoxyuridine (EdU) assay. Analysis differentiation (MyoD, MyoG, MYH2) immunofluorescence Myosin heavy chain (MyHC) used to assess analysis revealed inhibited primary myoblast. Furthermore, demonstrated promoted luciferase screening RNA immunoprecipitation (RIP) assays could have interaction with miR-2400. Additionally, miR-2400 myoblast, may eliminate effects miR-2400, as showed rescue experiments. Together, our results novel circular inhibit promote
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ژورنال
عنوان ژورنال: Cells
سال: 2021
ISSN: ['2073-4409']
DOI: https://doi.org/10.3390/cells10010176